L-Glycerol-3-phosphate Oxidase
Maelezo
Glycerol-3-phosphate oxidase (EC 1.1.3.21) ni kimeng'enya ambacho huchochea mmenyuko wa kemikali..Enzyme hii ni ya familia ya oxidoreductases, haswa wale wanaofanya kazi kwenye kikundi cha wafadhili cha CH-OH na kipokezi cha oksijeni.Jina la utaratibu la darasa hili la enzyme ni sn-glycerol-3-phosphate:oksijeni 2-oxidoreductase.Majina mengine yanayotumika kawaida ni pamoja na glycerol phosphate oxidase, glycerol-1-phosphate oxidase, glycerol phosphate oxidase, L-alpha-glycerophosphate oxidase, alpha-glycerophosphate oxidase, na L-alpha-glycerol-3-phosphate oxidase.Enzyme hii inashiriki katika kimetaboliki ya glycerophospholipid.Inatumia cofactor moja, FAD.
Utaratibu wa majibu
L-Glycerol-3-phosphate + O2 →Dihydroxyacetonephosphate + H2O2
Maombi
Enzyme hii hutumiwa kugundua triglycerides ya enzymatic katika uchunguzi wa kimatibabu.
Vipimo
| Vipengee vya Mtihani | Vipimo |
| Mwonekano | Poda ya amorphous ya kijani ya njano, lyophilized |
| Shughuli | ≥6U/mg |
| Usafi(SDS-PAGE) | ≥90% |
| Umumunyifu (10mg poda/ml) | Wazi |
| Kikatalani | ≤0.001% |
| ATPase | ≤0.005% |
| Glucose oxidase | ≤0.01% |
| Cholesterol oxidase | ≤0.01% |
Usafirishaji na uhifadhi
Usafiri:Mazingira
Hifadhi:Hifadhi kwa -20°C(Muda mrefu), 2-8°C (muda mfupi)
Maisha ya kujaribu tena yaliyopendekezwa:1 mwaka
