Hexokinase (HK)
Maelezo
Tumia Hexokinase ili kubaini D-glucose, D-fructose, na D-sorbitol katika sampuli za utafiti wa chakula au kibiolojia.Kimeng'enya hiki pia hutumika kwa uchanganuzi wa saccharides nyingine ambazo zinaweza kubadilishwa kuwa glukosi au fructose, na hivyo ni muhimu katika majaribio ya glycosides nyingi.
Ikiwa Hexokinase itatumiwa pamoja na glukosi-6-fosfati dehydrogenase (G6P-DH)* (vipimo vya glukosi6-fosfati vinavyoundwa na Hexokinase), sampuli hazipaswi kuwa na viwango vya juu vya fosfeti kwani G6P-DH imezuiliwa kwa ushindani na fosfati.
Muundo wa Kemikali
Kanuni ya Mwitikio
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Vipimo
Vipengee vya Mtihani | Vipimo |
Maelezo | Nyeupe hadi njano kidogo poda ya amorphous, lyophilized |
Shughuli | ≥30U/mg |
Usafi(SDS-PAGE) | ≥90% |
Umumunyifu (10mg poda/ml) | Wazi |
Proteases | ≤0.01% |
ATPase | ≤0.03% |
Phosphoglucose isomerase | ≤0.001% |
Creatine phosphokinase | ≤0.001% |
Glucose-6-Phosphate dehydrogenase | ≤0.01% |
NADH/NADPH oxidase | ≤0.01% |
Usafirishaji na uhifadhi
Usafiri: Amazingira
Hifadhi:Hifadhi kwa -20°C(Muda mrefu), 2-8°C (muda mfupi)
Jaribio upya linalopendekezwaMaisha:1 mwaka