D-Lactate dehydrogenase (LDH)
Maelezo
Enzyme hutumiwa kwa ajili ya kuondolewa kwa pyruvate katika maamuzi ya kufanya kazi na NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Matumizi
1. phosphatase ya alkali pamoja na protini (kingamwili, streptavidin nk,) inaweza kutambua hasa molekuli lengwa, na inaweza kutumika katika utambuzi wa ELISA, WB na histokemikali;
2. phosphatase ya alkali inaweza kutumika kuondoa phosphosphorize '-terminal 5 ya DNA au RNA ili kuzuia kujiunganisha;
3. DNA ya dephosphorylated hapo juu au RNA inaweza kuwekewa lebo na fosfati zenye lebo ya redio (kupitia T4 poly-nucleotide kinase)
Muundo wa Kemikali
Utaratibu wa majibu
D-Lactate + NAD+→ Pyruvate +NADH + H+
Vipimo
| Vipengee vya Mtihani | Vipimo |
| Maelezo | Poda nyeupe ya amorphous, lyophilized |
| Shughuli | ≥200U/mg |
| Usafi(SDS-PAGE) | ≥90% |
| Umumunyifu (10mg poda/mL) | Wazi |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Usafirishaji na uhifadhi
Usafiri:AmbIent
Hifadhi:Hifadhi kwa -20°C(Muda mrefu), 2-8°C (muda mfupi)
Jaribio upya linalopendekezwaMaisha:2 mwaka
